Experiences of investigators
We apply innovative technologies and have extensive expertise in isolating and cultivating primary cells, e.g. human mesenchymal stromal/stem cells (MSCs), other progenitor and differentiated cells, their characterization, expansion, manipulation and implementation of innovative regenerative technologies.
We have developed detailed protocols and standard operating procedures for the isolation and characterization of MSCs and other cells from a variety of human tissues, including bone marrow, articular cartilage, adipose tissue, cardiac muscle, skeletal muscle, menstrual blood and synovial fluid. We have extensive knowledge and experience of a wide range of morphological and functional assays for the characterisation of adult MSCs and progenitors and differentiated cells, as well as induced pluripotent stem cells.
Our current research is done to understand the biological properties of these cells and their potential for clinical applications, including possible mechanisms of action, safety and targeted regeneration. We have the capacity for establishing ex-vivo models of human cartilage, synovial tissue and cultures of isolated chondrons. We employ electric and mechanical stimulation and the analysis of mechanotransduction pathways well is within range of our capacity and scientific expertise.
Current research topics focus on degenerative and inflammatory diseases and cellular therapies with particular emphasis on regenerative medicine for articular cartilage, cardiac and lung tissues.
Last updated: 2023-09-18, 21:43:37
Fields of the investigation
Principal research topics at the Department of Regenerative Medicine include: studies on the capabilities of cellular therapies for osteoarthritis and cartilage defects, heart and skeletal muscle, chronic obstructive pulmonary disease (COPD) and other degenerative lung diseases, arrhythmias, autoimmune, inflammatory and other diseases; mesenchymal stem cell -based therapies and their cultures in 3D scaffolds for engineering of cartilage, lung, muscle and cardiac tissues, pacemaking technologies; investigation and characterization of aggressive cells in rheumatoid synovium and progenitor cells in cartilage; mechanotransduction and calcium signaling in cartilage and cardiac cells, effects of calcium channel inhibitors, epigenetic changes, including methyl, acetyl and other groups, modifying agents and their effect to the cells; application of photodynamic therapy, nanomedicine and others.
The search of disease biomarkers for osteoarthritis and development of multiplexed immunodetection nanobiosensor are the important fields of our interests at the department. We also work in the area of respiratory health assessing mechanisms of injury, i.e. cigarette smoking, air pollution etc., designing novel models and markers for in vitro studies and investigating novel candidate therapies.