Department of regenerative medicine

Eiva Bernotienė

Head of the Department

Fast-aging society drives state decision- makers and scientists to speed up their efforts in health care, especially in repairing, regenerating and reconstructing of lost tissues and designing new therapeutic strategies for organ-devastating diseases.
Relevant technical facilities together with high intellectual potential are concentrated at the Department of Regenerative Medicine of the Joint Center of Innovative Medicine. The Institution employs experienced stem cell researchers, immunologists and pathologists, have numerous contacts and collaborations worldwide, and participate in joint international projects. High level of clinically-relevant research, a range of employed specialists and collaboration with laser physicists, surgeons, rheumatologists and other professionals makes our Institution a reliable partner to join multidiscipline projects.

Experiences of investigators

Researchers at the Department of Regenerative Medicine are experienced in isolation and characterization of mesenchymal stem (stromal) cells (MSC) from different human tissues, including bone marrow, muscle, adipose tissue, human umbilical cord blood and placenta as well as cells from cartilage, muscle, synovial fluid and synovial tissue. A wide range of characteristics of those cells have been investigated seeking to understand their properties and potential, including safety and mechanisms of action, for the use in possible clinical translational applications.

 

Fields of the investigation


Principal topics of current research carried out at the Department of Regenerative Medicine and expertise include: analysis of stem cell application for the treatment of osteoarthritis, heart and skeletal muscle, chronic obstructive pulmonary disease (COPD), malfunction of pacemaker, autoimmune, inflammatory and other diseases; MSC cultures in 3D scaffolds for engineering of cartilage, lung, muscle and cardiac pacemaker tissues; investigation and characterization of aggressive cells in rheumatoid synovium and progenitor cells in cartilage; effects of epigenetically modifying agents and methyl group donors on the cells; application of photodynamic therapy, nanomedicine and others. We are very much interested in participation in the projects on relevant topics.

Department of Regenerative medicine is experienced in isolation of stem cells (particularly human mesenchymal, MSC), their characterization and optimization of growth conditions and technologies. We have already isolated and characterized MSC from different human tissues, including bone marrow and the alternative sources such as human umbilical cord blood and tissues; heart and skeletal muscle, placenta tissue; adipose tissue; cartilage; synovial fluid and synovial tissues. Different characteristics of those cells have been investigated seeking to understand their properties and potential, including safety and mechanism of action in possible clinical applications. Researchers have been involved in FP7 project ADIPOA 2010-2014. Topics of the research in this and other projects are associated with degenerative and inflammatory diseases and regeneration possibilities of stromal stem cells with particular interest in articular tissues. Current research topics include investigation of MSC for treatment of osteoarthritis, heart and lung diseases; MSC cultures in 3D bioscaffolds; search of aggressive cells and progenitors in synovium and cartilage; possibility of photodynamic therapy and application for the synovitis treatment in patients with rheumatoid arthritis; and many other various investigations.

 

Research equipments

The Department of Regenerative Medicine is well-equipped with the cell culture facilities, including two CO2 incubators (O2 controlled), mechanical pressure generator (Flexcell), flow cytometer/sorter (FACS Aria), fluorescent and light microscopes, laboratory of histology and immunohistochemistry, equipment for PCR, RTq-PCR, Western Blotting, 2D electrophoresis, cell electroporation, luminescent detection system (Luminex), plate-reader “Spectramax” for measurement of absorbance, fluorescence and luminescence, magnetic particle-based cell sorter (MACS), ultra low temperature freezer (-150˚C), etc.

 

Contacts:

Head of the department of Regenerative medicine: dr. Eiva Bernotiene. Phone: (8 5) 2626049

The addresses of departmental employees are based on the principle: name.surname@imcentras.lt

Zygmunt Mackevič

Chief Researcher

Edvardas Bagdonas

Researcher

Daiva Bironaitė

Senior Researcher

Aušra Ungurytė

Researcher

phD, prof. Ali Mobasheri

Senior Researcher

Ieva Bružauskaitė

Researcher
Dr. Eiva Bernotienė Head of Department, Senior Researcher
Prof. HDr. Zygmunt Mackevič Chief Researcher
Dr. Edvardas Bagdonas Researcher
Dr. Rūta Aldonytė  Researcher
Dr. Daiva Bironaitė Researcher
Dr. Sandra Meidutė-Abaravičienė Researcher
Dr. Aušra Ungurytė Researcher
Jaroslav Denkovskij Junior Researcher
 Vilma Jeršovienė Junior Researcher
Jovilė Raudoniūtė Junior Researcher, PhD candidate
Ieva Bružauskaitė Junior Researcher, PhD candidate
Ilona Uzielienė PhD candidate