Biopharmacy department

prof. habil. dr. Vladas Algirdas Bumelis

Head of Department, Chief Researcher

Biopharmacy Department conducts applied scientific researches of recombinant proteins and development of medical products manufacture technologies. The Researchers of Biopharmacy Department works on two main directions:

  • Improvement of biosynthesis processes and development of new biosynthesis technologies.
  • Development of the stem cells cultivation technologies and its application according Good Manufacturing Practice.

The two different expression systems: bacterial and mammal are used for the recombinant proteins production in first direction of the researches. In order to create expression system, the optimal transfection conditions are prepared. The selection of clones is made to create cells line bank, which efficiently produces target proteins. Selected cell clones are used for biosynthesis schemes creation in different volume bioreactors. The purification processes of recombinant proteins from bioreactors are optimized due to physiochemical properties of protein and cells line internals. The analytical methods for recombinant proteins received during biosynthesis process evaluation are improved in Biopharmacy Department.

The two different expression systems: bacterial and mammal are used for the recombinant proteins production in first direction of the researches. In order to create expression system, the optimal transfection conditions are prepared. The selection of clones is made to create cells line bank, which efficiently produces target proteins. Selected cell clones are used for biosynthesis schemes creation in different volume bioreactors. The purification processes of recombinant proteins from bioreactors are optimized due to physiochemical properties of protein and cells line internals. The analytical methods for recombinant proteins received during biosynthesis process evaluation are improved in Biopharmacy Department.

 

Scientific research topics:

  1. Research wokr of recombinant proteins expression systems, biosynthesis, purification and proteins properties. Topic is analyzed by prof. habil. dr. V.A. Bumelis, dr. G. Vyšniauskis, I. Ryžakovas, A. Kinderis, A. Pažusytė.
  2. Development of the stem cells of different origin cultivation technologies according to GGP. Topic is analyzed by prof. habil. dr. V.A. Bumelis, dr. G. Ramanauskaitė, A. Vitlipaitė.

 

APC services are provided in two laboratories – Biopharmaceutical Processes Research Laboratory and Disease Biomarkers Research Laboratory in Biopharmacy Department. The equipment in these laboratories are used for different activities in order to develop biopharmaceutical processes:

  1. The package for genetic material preparation, amplification, fractionation used for recombinant cells lines generation and biosynthesis processes creation.
  2. A set of biopharmaceutical processes research equipment for cells visualization to investigate the concentration and vitality of cells.
  3. The package of recombinant cells equipment for biosynthesis processes development and obtainment of final product.
  4. A set for recombinant proteins excretion and purification in order to get the final product.
  5. CO2 incubator, incubator-shave, centrifuge for routine works with cells cultures.
  6. Cells visualization system for recombinant cells lines analysis.
  7. Analyzer of glucose for biosynthesis processes optimization.
  8. System of fluids chromatography for large amounts of proteins cleaning.
  9. System of recombinant protein for yield measurement.
  10. Mass spectrometry and HPLC system are used in order to get characteristics of final product.
  11. The system for the analysis of proteins aggregates and particles, homogenizator, viscosimeter with temperature control, spectrometer for recombinant proteins purification schemes development and characterization.
  12. System for the sequencing of proteins for product characteristics.
  13. Liofilizator for final product preservation.

Gintautas Vyšniauskis

Researcher